Concentration10u/μl5 " . . . G↓G TA C C . . . 3 "3 " . . . C C AT G↑G . . . 5 "

Storage Buffer10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol

Unit Definition10u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation65˚C for 20 minutes.

Quality Control AssaysLigation/ Recutting AssayAfter 10-fold overdigestion with Acc65 I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Acc65 I for 16 hours at 37˚C.

Ordering Information

DownloadsManualAcc65 I (Kpn I*)

Vivantis是一家基于研究的生物技术公司，成立于2002年。我们专门生产限制性酶，DNA提取试剂盒，DNA扩增试剂和其他相关产品，用于分子生物学研究。我们的产品源于内部研究，旨在为全球生命科学研究人员提供集成解决方案。我们确保研发团队中经验丰富的科学家不断提供开拓性创新。这包括用于分子生物学应用的试剂盒，重组酶，改良的蛋白染色剂，标记和阶梯。