Concentration1-3u/μl5"…AA↓CGTT…3"3"…TTGC↑AA…5"

Reaction Conditions 1X Buffer V5 30mM Tris-acetate (pH7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 0.05% Triton X-100, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation65°C for 20 minutes.

Ligation / Recutting Assay After 3-fold overdigestion with Acl I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 1.5u of Acl I for 16 hours at 37°C.Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)*Blocked by CpG methylation

Ordering Information

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Vivantis是一家基于研究的生物技术公司，成立于2002年。我们专门生产限制性酶，DNA提取试剂盒，DNA扩增试剂和其他相关产品，用于分子生物学研究。我们的产品源于内部研究，旨在为全球生命科学研究人员提供集成解决方案。我们确保研发团队中经验丰富的科学家不断提供开拓性创新。这包括用于分子生物学应用的试剂盒，重组酶，改良的蛋白染色剂，标记和阶梯。