Concentration5-20u/μl5"…CCG↓CTC…3" 3"…GGC↑GAG…5"
Reaction Conditions 1X Buffer V5 30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation65°C for 20 minutes
Ligation / Recutting Assay After 5-fold overdigestion with AccBSI, 90% of the DNA fragments can be ligated and of these 50% can be recut.
Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AccBSI for 16 hours at 37°C.Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
Catalog No | Description | Pack Size |
RE1110 | AccBSI {BsrBI} | 500u |
DownloadsManualAccBSI {BsrBI}
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